Protein analysis

Glycan

Innovative Technology Support Group for Life Science and Drug Discovery

PC4-1

PC6-2 : Monosaccharide Composition Analysis

Test Introduction

A powerful technique to help you identify site-specific N-Glycan profile in your biopharmaceutical product. Determination of structure and localization of various glycan linked to asparagine in target proteins using LC-MS glycopeptide mapping techniques.

Sample

  • Biosimilar
  • Biobetter
  • Antibody
  • Antibody-drug conjugates
  • Recombinant protein drug
  • Toxin
  • vaccine

Analysis Process

  • Acid Hydrolysis
  • Bio-LC
  • Characterization of peaks
  • Quantitative analysis

Analysis Result

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Samples/sugars Recombinant Protein
fucose 2.5
galactose 5.2
mannose 17.0
galactosamine 0.5
glucosamine 9.4
N-acetylneuraminic acid 2.1
N-glycolylneuraminic acid 0.1
Sugar (%, w/w) 3.7
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No. Ret.Time min Peak Name Height nC Area nC*min symmetry(EF) Amount pmol esolution(EF)
1 4.98 Fucose 49.505 9.379 1.19 488.606 9.000
2 8.48 Galactosamine 61.790 19.155 1.11 488.070 2.83
3 10.00 Glucosamine 41.979 15.850 1.06 488.978 3.60
4 12.27 Galactose 35.286 15.203 n.a. 498.961 1.48
5 13.28 Glucose 33.069 14.774 n.a. 497.570 1.54
6 14.42 Mannose 22.548 10.828 1.14 490.714 n.a.
Total: 244.177 85.189 4.50 2952.899

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No. Ret.Time min Peak Name Height nC Area nC*min symmetry(EF) Amount pmol esolution(EF)
1 4.97 Fucose 3.093 0.565 1.19 29.452 6.61
2 8.28 n.a. 2.133 0.926 1.06 n.a. 2.62
3 9.98 Glucosamine 14.741 5.588 0.97 172.408 3.68
4 12.27 Galactose 4.386 1.830 1.06 60.055 1.50
5 13.28 Glucose 2.987 1.276 1.10 42.991 1.53
6 14.40 Mannose 8.410 4.091 1.14 185.409 n.a.
Total: 35.750 14.277 6.53 490.315